EDIM® technology is a form of “immunologic biopsy” for the detection of specific antigens in immune cells. The use of the immune system allows exceptional accuracy for diagnostics.
EDIM® technology detects disease-specific epitopes inside markophage
(Epitope Detection in Monocytes).
EDIM® technology screens macrophages for antigens that have previously been taken up into the cell interior by these immune cells through phagocytosis – which is why we refer to it as immunologic biopsy. For this purpose, CD14 and CD16 surface antibodies are used, which bind specifically to the surface of the cells. The cells are then briefly permeabilized to allow additional antibodies to enter the cells. These antibodies are each directed against the antigens that are to be detected by the method.
In the case of PanTum Detect, for example, TKTL1 and DNaseX (Apo10) antibodies are introduced into cells. Cell structures on the surface of cells can be detected very efficiently in blood samples using dye-labeled antibodies and subsequent detection of these dyes using laser beams in a flow cytometry procedure. This makes it possible to detect, count and further characterize phagocytes in blood. In this way, signals originating from bound antibodies inside the cells are also detected. This makes it possible to detect what the scavenger cell has recognized and eliminated by phagocytosis. For example, if tumor cells are present in the organism in the case of a tumor disease, the system detects signals from the dyes coupled to the TKTL1 and DNaseX (Apo10) antibodies.
Li Y.; Xu C.; Wang B.; Xu F.; Ma F. et al.: Proteomic characterization of gastric cancer response to chemotherapy and targeted therapy reveals new therapeutic strategies. nature communications 2022
Pinson A.; Xing L.; Xinnan L.; Namba T.; Kalebic N; Peters J. et al.: Human TKTL1 implies greater neurogenesis in frontal neocortex of modern humans than Neanderthals. Science 2022
Liu Q.; Fangming Z.; Xinnan L.; Ying L.; Ke Y.; Na T.; et al.: Non-oxidative pentose phosphate pathway controls regulatory T cell function by integrating metabolism and epigenetics. nature metabolism 2022
Hao S.; Qingfei, M.; Huihui S.; Yunkuo L.; Yao L.; et al.: The role of transketolase in human cancer progression and therapy. Elsevier 2022
Urla, C.; Stagno, M.J.; Schmidt, A.; Handgretinger, R.; Fuchs, J.; Warmann, S.W.; Schmid, E. Epitope Detection in Monocytes (EDIM) As a New Method of Liquid Biopsy in Pediatric Rhabdomyosarcoma. Biomedicines 2022, 10, 1812.
Burg S, Grust ALC, Feyen O, Failing K, Banat G-A, Coy JF, et al. Blood-Test Based Targeted Visualization Enables Early Detection of Premalignant and Malignant Tumors in Asymptomatic Individuals. Journal of Clinical and Medical Images. 2022;6.
Stagno M, Schmidt A, Bochem J, et al. Epitope detection in monocytes (EDIM) for liquid biopsy including identification of GD2 in childhood neuroblastoma—a pilot study. British Journal of Cancer. 2022;7.
Baptista I, Karakitsou E, Cazier J-B, Günther UL, Marin S, Cascante M. TKTL1 Knockdown Impairs Hypoxia-Induced Glucose-6-phosphate Dehydrogenase and Glyceraldehyde-3-phosphate Dehydrogenase Overexpression. Int J Mol Sci. 2022;23:3574.
Li M, Zhao X, Yong H, Xu J, Qu P, Qiao S, et al. Transketolase promotes colorectal cancer metastasis through regulating AKT phosphorylation. Cell Death Dis. 2022;13:99.
Niu C, Qiu W, Li X, Li H, Zhou J, Zhu H. Transketolase Serves as a Biomarker for Poor Prognosis in Human Lung Adenocarcinoma. J Cancer. 2022;13:2584–93.
Zhang L, Huang Z, Cai Q, Zhao C, Xiao Y, Quan X, et al. Inhibition of Transketolase Improves the Prognosis of Colorectal Cancer. Front Med. 2022;9:837143.