Research & Development


The pioneering importance of the biomarkers TKTL1 and Apo10 is very impressively demonstrated through studies by renowned research groups. These markers are detectable using the EDIM technology developed by Zyagnum and help with many oncological questions. We have compiled an overview of the relevant publications for you.


Coy JF: EDIM-TKTL1/Apo10 Blood Test: An Innate Immune System Based Liquid Biopsy for the Early Detection, Characterization and Targeted Treatment of Cancer. Int J Mol Sci. 2017 Apr 20;18(4). pii: E878. doi: 10.3390/ijms18040878. [PubMed]

Dong, Y.; Wang, M. Knockdown of TKTL1 additively complements cisplatin-induced cytotoxicity
in nasopharyngeal carcinoma cells by regulating the levels of NADPH and ribose-5-phosphate.
Biomed. Pharmacother. 2017, 85, 672–678. [PubMed]


Grimm, M.; Hoefert, S.; Krimmel, M.; Biegner, T.; Feyen, O.; Teriete, P.; Reinert, S. Monitoring carcinogenesis in a case of oral squamous cell carcinoma using a panel of new metabolic blood biomarkers as liquid biopsies. Oral Maxillofac Surg. 2016 Sep;20(3):295-302 [CrossRef] [PubMed]

Diaz-Moralli, S.; Aguilar, E.; Marin, S.; Coy, J.F.; Dewerchin, M.; Antoniewicz, M.R.; Meca-Cortés, O.; Notebaert, L.; Ghesquière, B.; Eelen, G.; et al. A key role for Transketolase-like 1 in tumor metabolic reprogramming. Oncotarget. 2016 Aug 9;7(32):51875-51897. [CrossRef] [PubMed]

Grimm, M.; Feyen, O.; Coy, J.F.; Hofmann, H.; Teriete, P.; Reinert, S. Analysis of circulating CD14+/CD16+ monocyte-derived macrophages (MDMs) in the peripheral blood of patients with oral squamous cell carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Mar;121(3):301-6. [CrossRef] [PubMed]

Jayachandran, A.; Lo, P.H.; Chueh, A.C.; Prithviraj, P.; Molania, R.; Davalos-Salas, M.; Anaka, M.; Walkiewicz, M.; Cebon, J.; Behren, A. Transketolase-like 1 ectopic expression is associated with DNA hypomethylation and induces theWarburg effect in melanoma cells. BMC Cancer. 2016 Feb 22;16:134. [CrossRef]

Xu, I.M.; Lai, R.K.; Lin, S.H.; Tse, A.P.; Chiu, D.K.; Koh, H.Y.; Law, C.T.; Wong, C.M.; Cai, Z.; Wong, C.C.;
et al. Transketolase counteracts oxidative stress to drive cancer development. Proc Natl Acad Sci U S A. 2016 Feb 9;113(6):E725-34. [CrossRef] [PubMed]

Grimm, M.; Kraut, W.; Hoefert, S.; Krimmel, M.; Biegner, T.; Teriete, P.; Cetindis, M.; Polligkeit, J.; Kluba, S.; Munz, A.; et al. Evaluation of a biomarker based blood test for monitoring surgical resection of oral squamous cell carcinomas.Clin. Oral Investig. 2016, 20, 329–338. [CrossRef] [PubMed]

Ahopelto, K.; Böckelman, C.; Hagström, J.; Koskensalo, S.; Haglund, C. Transketolase-like protein 1 expression predicts poor prognosis in colorectal cancer. Cancer Biol. Ther. 2016, 17, 163–168. [CrossRef] [PubMed]


Song, Y.; Liu, D.; He, G. TKTL1 and p63 are biomarkers for the poor prognosis of gastric cancer patients. Cancer Biomark. 2015, 15, 591–597. [CrossRef] [PubMed]

Li, J.; Zhu, S.C.; Li, S.G.; Zhao, Y.; Xu, J.R.; Song, C.Y. TKTL1 promotes cell proliferation and metastasis in esophageal squamous cell carcinomaBiomed Pharmacother. 2015 Aug;74:71-6. [CrossRef] [PubMed]

Ricciardelli, C.; Lokman, N.A.; Cheruvu, S.; Tan, I.A.; Ween, M.P.; Pyragius, C.E.; Ruszkiewicz, A.; Hoffmann, P.; Oehler, M.K. Transketolase is upregulated in metastatic peritoneal implants and promotes ovarian cancer cell proliferation. Clin. Exp. Clin Exp Metastasis. 2015 Jun;32(5):441-55. [CrossRef] [PubMed]


Li, B.; Iglesias-Pedraz, J.M.; Chen, L.Y.; Yin, F.; Cadenas, E.; Reddy, S.; Comai, L. Downregulation of the Werner syndrome protein induces a metabolic shift that compromises redox homeostasis and limits proliferation of cancer cells. Aging Cell 2014, Apr. 13, 367–378. [CrossRef] [PubMed]


Grimm M et al: A biomarker based detection and characterization of carcinomas exploiting two fundamental biophysical mechanisms in mammalian cells. BMC Cancer. 2013 Dec 4;13:569.Universitätsklinikum Tübingen, DKFZ Heidelberg [PubMed]

Grimm M et al: Transketolase-like protein 1 expression in recurrent oral squamous cell carcinoma after curative resection: a case report. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Sep;116(3):e173-8. [PubMed]

Jansen N und Coy JF: Diagnostic use of EDIM-blood test for early detection of colon cancer metastasis. Future Oncol. 2013 Apr;9(4):605–9. [PubMed]

Bentz S et al: Hypoxia induces the expression of transketolase-like 1 in human colorectal cancer. Digestion. 2013;88(3):182-92. Universitätsspital Zürich [PubMed]

Rotmann A: The use of new diagnostic tests for the monitoring of new and existing therapies for breast cancer patients. J. Clin. Oncol., ASCO Meeting Abstracts. 2013; 31:e22006


Feyen O et al: EDIM-TKTL1 blood test: a noninvasive method to detect upregulated glucose metabolism in patients with malignancies. Future Oncol. 2012 Oct;8(10):1349-59. Rhönklinikum Bad Berka [PubMed]

Feyen O et al: EDIM blood test: a non-invasive method to detect patients with malignancies. Cell Symposia July 6-8, 2012: Poster

Lange CA et al: Enhanced TKTL1 Expression in Malignant Tumors of the Ocular Adnexa Predicts Clinical Outcome. Ophthalmology. 2012 Sep;119(9):1924-9. Epub 2012 Jun 1. Universitätsklinikum Freiburg [PubMed]

Wanka C et al: Tp53-induced glycolysis and apoptosis regulator (TIGAR) protects glioma cells from starvationinduced cell death by upregulating respiration and improving cellular redox homeostasis. J Biol Chem. 2012 Sep 28;287(40):33436-46. Epub 2012 Aug 10. [PubMed]

Wani K et al: A prognostic gene expression signature in infratentorial ependymoma. For the Collaborative Ependymoma Research Network. Acta Neuropathol. 2012 May;123(5):727-38. Epub 2012 Feb 10. [PubMed]


Kayser G et al: Poor outcome in primary non-small cell lung cancers is predicted by transketolase TKTL1 expression. Pathology. 2011 Dec;43(7):719-24. Universitätsklinikum Freiburg [PubMed]

Schwaab J et al: Expression of Transketolase like gene 1 (TKTL1) predicts disease-free survival in patients with locally advanced rectal cancer receiving neoadjuvant chemoradiotherapy. BMC Cancer 2011;11:363. Universitätsmedizin Mannheim [PubMed]

Diaz-Moralli S et al: Transketolase-like 1 expression is modulated during colorectal cancer progression and metastasis formation. PLoS One. 2011;6(9):e25323. Epub 2011 Sep 27 [PubMed]

Hartmannsberger D et al: Transketolase-like protein 1 confers resistance to serum withdrawal in vitro. Cancer Lett. 2011 Jan 1;300(1):20-9. Epub 2010 Sep 29. Universitätsklinikum München


Krockenberger M et al: Expression of transketolase-like 1 protein (TKTL1) in human endometrial cancer. Anticancer Res. 2010 May;30(5):1653-9. Universitätsklinikum Würzburg [PubMed]

Yuan W et al: Silencing of TKTL1 by siRNA inhibits proliferation of human gastric cancer cells in vitro and in vivo. Cancer Biol Ther. 2010 May;9(9):710-6. Epub 2010 May [PubMed]

Kayser G et al: Lactate-dehydrogenase 5 is overexpressed in non-small cell lung cancer and correlates with the expression of the transketolase-like protein 1. Diagn Pathol. 2010 Apr 12;5:22. Universitätsklinikum Freiburg [PubMed]

Sun W et al: TKTL1 is activated by promoter hypomethylation and contributes to head and neck squamous cell carcinoma carcinogenesis through increased aerobic glycolysis and HIF1alpha stabilization. Clin Cancer Res. 2010 Feb 1;16(3):857-66. Epub 2010 Jan 26 [PubMed]

Schmidt M et al: Glycolytic phenotype in breast cancer: activation of Akt, up-regulation of GLUT1, TKTL1 and down-regulation of M2PK. J Cancer Res Clin Oncol. 2010 Feb;136(2):219-25. Epub 2009 Aug 5. Universitätsklinikum Würzburg [PubMed]


Wu HT et al: Anaplastic nephroblastomas express transketolase-like enzyme 1. J Clin Pathol. 2009 May;62(5):460-3. Epub 2009 Jan 12 [PubMed]

Chen H et al: Overexpression of transketolase-like gene 1 is associated with cell proliferation in uterine cervix cancer. J Exp Clin Cancer Res. 2009 Mar 30;28:43. [PubMed]

Smith IM et al: Coordinated activation of candidate proto-oncogenes and cancer testes antigens via promoter demethylation in head and neck cancer and lung cancer. PLoS One. 2009;4(3):e4961. Epub 2009 Mar 23 [PubMed]

Xu X et al: Transketolase-like protein 1 (TKTL1) is required for rapid cell growth and full viability of human tumor cells. Int J Cancer. 2009 Mar 15;124(6):1330-7. Deutsches Krebsforschungszentrum Heidelberg [PubMed]


Zerilli M et al: Increased expression of transketolase-like-1 in papillary thyroid carcinomas smaller than 1.5 cm in diameter is associated with lymph-node metastases. Cancer. 2008 Sep 1;113(5):936-44 [PubMed]

Völker HU et al: Expression of transketolase-like 1 and activation of Akt in grade IV glioblastomas compared with grades II and III astrocytic gliomas. Am J Clin Pathol. 2008 Jul;130(1):50-7. Universitätsklinikum Würzburg [PubMed]

Kohrenhagen N et al: Expression of transketolase-like 1 (TKTL1) and p-Akt correlates with the progression of cervical neoplasia. J Obstet Gynaecol Res. 2008 Jun;34(3):293-300. Universitätsklinikum Würzburg [PubMed]

Langbein S et al: Metastasis is promoted by a bioenergetic switch: new targets for progressive renal cell cancer. Int J Cancer. 2008 Jun 1;122(11):2422-8 [PubMed]

Schmidt M et al: Glucose metabolism and angiogenesis in granulosa cell tumors of the ovary: activation of Akt, expression of M2PK, TKTL1 and VEGF. Eur J Obstet Gynecol Reprod Biol. 2008 Jul;139(1):72-8. Epub 2008 Apr 3. Universitätsklinikum Würzburg [PubMed]


Völker HU et al: Overexpression of transketolase TKTL1 is associated with shorter survival in laryngeal squamous cell carcinomas. Eur Arch Otorhinolaryngol. 2007 Dec;264(12):1431-6. Epub 2007 Jul 18. Universitätsklinikum Würzburg [Abstract]

Földi M et al: Transketolase protein TKTL1 overexpression: A potential biomarker and therapeutic target in breast cancer. Oncol Rep. 2007 Apr;17(4):841-5. Universitätsklinikum Freiburg [PubMed]

Krockenberger M et al: Transketolase-like 1 expression correlates with subtypes of ovarian cancer and the presence of distant metastases. Int J Gynecol Cancer 2007 Jan-Feb;17(1):101-6. Universitätsklinikum Würzburg [PubMed]


Langbein S et al: Expression of transketolase TKTL1 predicts colon and urothelial cancer patient survival: Warburg effect reinterpreted. Br J Cancer. 2006 Feb 27;94(4):578-85. Universitätsmedizin Mannheim [PubMed]

Staiger WI et al: Expression of the mutated transketolase TKTL1, a molecular marker in gastric cancer. Oncol Rep. 2006 Oct;16(4):657-61. Universitätsmedizin Mannheim [PubMed]

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